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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
13/11/2015 |
Actualizado : |
13/11/2015 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
LADO, J.; CRONJE, P.; ALQUÉZAR, B.; PAGE, A.; MANZI, M.; GÓMEZ-CADENAS, A.; STEAD, A.D.; ZACARÍAS, L.; RODRIGO, M.J. |
Afiliación : |
JOANNA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Fruit shading enhances peel color, carotenes accumulation and chromoplast differentiation in red grapefruit. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
Physiologia Plantarum, 2015, v.154, no. 4, p. 469-484. |
Serie : |
0031-9317 |
DOI : |
10.1111/ppl.12332 |
Idioma : |
Inglés |
Contenido : |
ABSTRACT.
The distinctive color of red grapefruits is due to lycopene, an unusual carotene in citrus. It has been observed that red ?Star Ruby? (SR) grapefruits grown inside the tree canopy develop a more intense red coloration than those exposed to higher light intensities. To investigate the effect of light on SR peel pigmentation, fruit were bagged or exposed to normal photoperiodic conditions, and changes in carotenoids, expression of carotenoid biosynthetic genes and plastid ultrastructure in the peel were analyzed. Light avoidance accelerated chlorophyll breakdown and induced carotenoid accumulation, rendering fruits with an intense coloration. Remarkably, lycopene levels in the peel of shaded fruits were 49-fold higher than in light-exposed fruit while concentrations of downstream metabolites were notably reduced, suggesting a bottleneck at the lycopene cyclization in the biosynthetic pathway. Paradoxically, this increment in carotenoids in covered fruit was not mirrored by changes in mRNA levels of carotenogenic genes, which were mostly up-regulated by light. In addition, covered fruits experienced profound changes in chromoplast differentiation, and the relative expression of genes related to chromoplast
development was enhanced. Ultrastructural analysis of plastids revealed an acceleration of chloroplasts to chromoplast transition in the peel of covered fruits concomitantly with development of lycopene crystals and plastoglobuli. In this sense, an accelerated differentiation of chromoplasts may provide biosynthetic capacity and a sink for carotenoids without involving major changes in transcript levels of carotenogenic genes. Light signals seem to regulate carotenoid accumulation at the molecular and structural level by
influencing both biosynthetic capacity and sink strength. Abbreviations ? 𝛽CHX, 𝛽-carotene hydroxylase; 𝛽LCY, lycopene cyclase 𝛽; ABA, abscisic acid; C, covered; Chl, chlorophyll; DXS, 1-deoxy-D-xylulose-5-phosphate synthase; FIB, fibrillin; FW, fresh weight; GGPP, geranyl geranyl pyrophosphate; GGPPS, geranyl geranyl pyrophosphate synthase; HDR, hydroxymethylbutenyl diphosphate reductase; HPLC, high-performance liquid chromatography; MEP, methyl-D-erythritol-4-phosphate; NC, non-covered; PCR, polymerase chain reaction; PDS, phytoene desaturase; PSY, phytoene synthase; sHSP, small heat shock protein; SR, Star Ruby; ZDS, 𝜁-carotene desaturase.
Physiol. Plant. MenosABSTRACT.
The distinctive color of red grapefruits is due to lycopene, an unusual carotene in citrus. It has been observed that red ?Star Ruby? (SR) grapefruits grown inside the tree canopy develop a more intense red coloration than those exposed to higher light intensities. To investigate the effect of light on SR peel pigmentation, fruit were bagged or exposed to normal photoperiodic conditions, and changes in carotenoids, expression of carotenoid biosynthetic genes and plastid ultrastructure in the peel were analyzed. Light avoidance accelerated chlorophyll breakdown and induced carotenoid accumulation, rendering fruits with an intense coloration. Remarkably, lycopene levels in the peel of shaded fruits were 49-fold higher than in light-exposed fruit while concentrations of downstream metabolites were notably reduced, suggesting a bottleneck at the lycopene cyclization in the biosynthetic pathway. Paradoxically, this increment in carotenoids in covered fruit was not mirrored by changes in mRNA levels of carotenogenic genes, which were mostly up-regulated by light. In addition, covered fruits experienced profound changes in chromoplast differentiation, and the relative expression of genes related to chromoplast
development was enhanced. Ultrastructural analysis of plastids revealed an acceleration of chloroplasts to chromoplast transition in the peel of covered fruits concomitantly with development of lycopene crystals and plastoglobuli. In this sense, an accelerated diff... Presentar Todo |
Thesagro : |
CITRUS; CITRUS PARADISI. |
Asunto categoría : |
-- |
Marc : |
LEADER 03223naa a2200265 a 4500 001 1053867 005 2015-11-13 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1111/ppl.12332$2DOI 100 1 $aLADO, J. 245 $aFruit shading enhances peel color, carotenes accumulation and chromoplast differentiation in red grapefruit.$h[electronic resource] 260 $c2015 490 $a0031-9317 520 $aABSTRACT. The distinctive color of red grapefruits is due to lycopene, an unusual carotene in citrus. It has been observed that red ?Star Ruby? (SR) grapefruits grown inside the tree canopy develop a more intense red coloration than those exposed to higher light intensities. To investigate the effect of light on SR peel pigmentation, fruit were bagged or exposed to normal photoperiodic conditions, and changes in carotenoids, expression of carotenoid biosynthetic genes and plastid ultrastructure in the peel were analyzed. Light avoidance accelerated chlorophyll breakdown and induced carotenoid accumulation, rendering fruits with an intense coloration. Remarkably, lycopene levels in the peel of shaded fruits were 49-fold higher than in light-exposed fruit while concentrations of downstream metabolites were notably reduced, suggesting a bottleneck at the lycopene cyclization in the biosynthetic pathway. Paradoxically, this increment in carotenoids in covered fruit was not mirrored by changes in mRNA levels of carotenogenic genes, which were mostly up-regulated by light. In addition, covered fruits experienced profound changes in chromoplast differentiation, and the relative expression of genes related to chromoplast development was enhanced. Ultrastructural analysis of plastids revealed an acceleration of chloroplasts to chromoplast transition in the peel of covered fruits concomitantly with development of lycopene crystals and plastoglobuli. In this sense, an accelerated differentiation of chromoplasts may provide biosynthetic capacity and a sink for carotenoids without involving major changes in transcript levels of carotenogenic genes. Light signals seem to regulate carotenoid accumulation at the molecular and structural level by influencing both biosynthetic capacity and sink strength. Abbreviations ? 𝛽CHX, 𝛽-carotene hydroxylase; 𝛽LCY, lycopene cyclase 𝛽; ABA, abscisic acid; C, covered; Chl, chlorophyll; DXS, 1-deoxy-D-xylulose-5-phosphate synthase; FIB, fibrillin; FW, fresh weight; GGPP, geranyl geranyl pyrophosphate; GGPPS, geranyl geranyl pyrophosphate synthase; HDR, hydroxymethylbutenyl diphosphate reductase; HPLC, high-performance liquid chromatography; MEP, methyl-D-erythritol-4-phosphate; NC, non-covered; PCR, polymerase chain reaction; PDS, phytoene desaturase; PSY, phytoene synthase; sHSP, small heat shock protein; SR, Star Ruby; ZDS, 𝜁-carotene desaturase. Physiol. Plant. 650 $aCITRUS 650 $aCITRUS PARADISI 700 1 $aCRONJE, P. 700 1 $aALQUÉZAR, B. 700 1 $aPAGE, A. 700 1 $aMANZI, M. 700 1 $aGÓMEZ-CADENAS, A. 700 1 $aSTEAD, A.D. 700 1 $aZACARÍAS, L. 700 1 $aRODRIGO, M.J. 773 $tPhysiologia Plantarum, 2015$gv.154, no. 4, p. 469-484.
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Las Brujas. |
Fecha actual : |
12/11/2015 |
Actualizado : |
19/11/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
SILVA, P.; CALVO-SALAZAR, V.; CONDON, F.; QUINCKE, M.; PRITSCH, C.; GUTIÉRREZ, L.; CASTRO, A.; HERRERA-FOESSEL, S.; VON ZITZEWITZ, J.; GERMAN, S. |
Afiliación : |
MARIA PAULA SILVA VILLELLA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIOLETA CALVO-SALAZAR, CIMMYT (Centro Internacional de Mejoramiento de Maíz y Trigo); FEDERICO CONDON PRIANO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN CONRADO QUINCKE WALDEN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CLARA PRITSCH, Universidad de la República (UdelaR)/ Facultad de Agronomía; LUCÍA GUTIÉRREZ, Universidad de la República (UdelaR)/ Facultad de Agronomía; ARIEL CASTRO, Universidad de la República (UdelaR)/ Facultad de Agronomía; SYBIL HERRERA-FOESSEL, CIMMYT (Centro Internacional de Mejoramiento de Maíz y Trigo); JARISLAV VON ZITEZWEITZ, SECOBRA; SILVIA ELISA GERMAN FAEDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Effects and interactions of genes Lr34, Lr68 and Sr2 on wheat leaf rust adult plant resistance in Uruguay |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
Euphytica, 2015, v. 204, p. 599?608. |
ISSN : |
1573-5060. |
DOI : |
10.1007/s10681-014-1343-6 |
Idioma : |
Inglés |
Contenido : |
ABSTRACT.
Achieving durable resistance to leaf rust (LR), caused by Puccinia triticina, in wheat has been one of the main objectives of breeding programs. Durability of LR resistance is considered to be associated with adult plant resistance (APR) genes that are quantitatively inherited and whose expression is largely influenced by environment. Our objective
was to study the effects and interactions of APR genes Lr34, Lr68 and Sr2 on LR response in Uruguay using two BC1F6 populations (?LE2304*2/Parula? and ?ORL99192*2/Parula?). The experimental material was screened for LR in three artificially inoculated environments (La Estanzuela 2012 and 2013 and Young 2012). Linked molecular markers were used to
detect the presence of the genes conferring resistance to LR. Gene Lr34 was fixed in the ?LE2304*2/Parula? population. Lr68?Sr2 resulted in a reduction of 79 % of the LR area under the disease progress curve (AUDPC), while the presence of Lr68 alone resulted in a reduction of 51 %. In the ?ORL99192*2/Parula? population, the combined effect of Lr34?Lr68?Sr2 led to the highest reduction of LR AUDPC (73 %). Individually, Lr34 and Lr68 resulted in a reduction of the LR AUDPC of 14 and 50 %, respectively. Sr2 alone did not reduce LR AUDPC but enhanced the effect of either Lr34 or Lr68. Unlike previous reports Lr68 conferred a higher level of LR resistance than Lr34 in Uruguay.
@Springer Science+Business Media Dordrecht 2015 |
Palabras claves : |
ADDITIVE RESISTANCE; ADULT PLANT RESISTANCE; DESARROLLO LENTO DE ROYA; GENES DE RESISTENCIA DE PLANTA ADULTA; GENES DE RESISTENCIA DE PLÁNTULAS; GENES MAYORES; GENES MENORES; PUCCINIA TRITICINA; ROYA DE LA HOJA DE TRIGO; SLOW RUSTING; SLOW RUSTING RESISTANCE; TRITICUM AESTIVUM. |
Thesagro : |
TRIGO; TRITICUM AESTIVUM. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
Marc : |
LEADER 02663naa a2200421 a 4500 001 1053859 005 2019-11-19 008 2015 bl uuuu u00u1 u #d 022 $a1573-5060. 024 7 $a10.1007/s10681-014-1343-6$2DOI 100 1 $aSILVA, P. 245 $aEffects and interactions of genes Lr34, Lr68 and Sr2 on wheat leaf rust adult plant resistance in Uruguay 260 $c2015 520 $aABSTRACT. Achieving durable resistance to leaf rust (LR), caused by Puccinia triticina, in wheat has been one of the main objectives of breeding programs. Durability of LR resistance is considered to be associated with adult plant resistance (APR) genes that are quantitatively inherited and whose expression is largely influenced by environment. Our objective was to study the effects and interactions of APR genes Lr34, Lr68 and Sr2 on LR response in Uruguay using two BC1F6 populations (?LE2304*2/Parula? and ?ORL99192*2/Parula?). The experimental material was screened for LR in three artificially inoculated environments (La Estanzuela 2012 and 2013 and Young 2012). Linked molecular markers were used to detect the presence of the genes conferring resistance to LR. Gene Lr34 was fixed in the ?LE2304*2/Parula? population. Lr68?Sr2 resulted in a reduction of 79 % of the LR area under the disease progress curve (AUDPC), while the presence of Lr68 alone resulted in a reduction of 51 %. In the ?ORL99192*2/Parula? population, the combined effect of Lr34?Lr68?Sr2 led to the highest reduction of LR AUDPC (73 %). Individually, Lr34 and Lr68 resulted in a reduction of the LR AUDPC of 14 and 50 %, respectively. Sr2 alone did not reduce LR AUDPC but enhanced the effect of either Lr34 or Lr68. Unlike previous reports Lr68 conferred a higher level of LR resistance than Lr34 in Uruguay. @Springer Science+Business Media Dordrecht 2015 650 $aTRIGO 650 $aTRITICUM AESTIVUM 653 $aADDITIVE RESISTANCE 653 $aADULT PLANT RESISTANCE 653 $aDESARROLLO LENTO DE ROYA 653 $aGENES DE RESISTENCIA DE PLANTA ADULTA 653 $aGENES DE RESISTENCIA DE PLÁNTULAS 653 $aGENES MAYORES 653 $aGENES MENORES 653 $aPUCCINIA TRITICINA 653 $aROYA DE LA HOJA DE TRIGO 653 $aSLOW RUSTING 653 $aSLOW RUSTING RESISTANCE 653 $aTRITICUM AESTIVUM 700 1 $aCALVO-SALAZAR, V. 700 1 $aCONDON, F. 700 1 $aQUINCKE, M. 700 1 $aPRITSCH, C. 700 1 $aGUTIÉRREZ, L. 700 1 $aCASTRO, A. 700 1 $aHERRERA-FOESSEL, S. 700 1 $aVON ZITZEWITZ, J. 700 1 $aGERMAN, S. 773 $tEuphytica, 2015$gv. 204, p. 599?608.
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